Premium Analysis of Yeast Cells, Organelles and Derived Samples

Product Description

Coverage:
This analysis provides a comprehensive coverage of 21 distinct lipid classes and additional free sterols coverage:

• PA – phosphatidate;
• PC – phosphatidylcholine;
• PE – phosphatidylethanolamine;
• PG – phosphatidylglycerol;
• PI – phosphatidylinositol;
• PS – phosphatidylserine;
• DAG – diacylglycerol;
• TAG – triacylglycerol;
• EE – ergosteryl ester;
• LPA – lyso-phosphatidate;
• LPC – lyso-phosphatidylcholine;
• LPE – lyso-phosphatidylethanolamine;
• LPG – lyso-phosphatidylglycerol;
• LPI – lyso-phosphatidylinositol;
• LPS – lyso-phosphatidylserine;
• CER – ceramide;
• CL – cardiolipin;
• IPC – inositolphosphorylceramide;
• MIPC – mannosyl-inositol-phosphorylceramide;
• M(IP)₂C – mannosyl-di-(inositolphosphoryl)-ceramide;
• CDP-DAG – CDP-diacylglycerol;

Free sterols coverage (optional):

• Erg – ergosterol (optional).

The exact number of discovered lipids provided in results depends on samples.

Analysis:
Each sample is extracted, acquired and analyzed once. We recommend to include multiple sample replicates in order to obtain statistically meaningful data.

Approach:
Untargeted high-resolution shotgun lipidomics-generated data analyzed using LipotypeXplorer, our proprietary software for identifying all measurable lipids using the molecular fragmentation query language (MFQL) and thus unimpeded by the traditional reliance on predefined spectral databases.

Results:
Results are fully quantitative and expressed in absolute values – pmol per sample volume and mol%. The absolute quantification is achieved using lipid class-specific internal standards. Lipotype will provide the data on sample volume analyzed and molar amounts of lipid molecules per sample. Please note: Lipotype will not and shall not be obliged to provide any Lipotype background intellectual property (including Lipotype know-how) or any data incorporating such Lipotype background intellectual property, notably no raw data will be provided.

Structural details:
With the advantage of MS2 fragmentation glycerophospholipids and glycerolipids (except TAG) are reported as subspecies, giving additional information on fatty acids – e.g. PI 18:1;0_16:0;0 denotes phosphatidylinositol with octadecenoic (18:1;0) and hexadecanoic (16:0;0) fatty acids, for which the exact position (sn-1 or sn-2) in relation to the glycerol backbone cannot be discriminated. PC O-18:1;0/16:0;0 denotes an ether- phosphatidylcholine, where an alkyl chain with 18 carbon atoms and 1 double bond (O-18:1;0) is ether-bound to sn-1 position of the glycerol and a hexadecanoic acid (16:0;0) is connected via an ester bond to the sn-2 position of the glycerol.The remaining lipid classes are reported as species e.g. – TAG 56:3;0.

Sample amount adjustment:
Despite the broad dynamic range of our analytical methods, we pre-test the total lipid amount occurring in submitted samples to ensure that appropriate amounts are used in order to allow for or increase the analysis quality and result comparability. The customer will be presented with options to continue, discontinue or sending new samples if this pre-test proves that samples are not suitable to perform the analysis.

Report:
The results are delivered in the form of a Standard Report containing information on methodology, analysis details, used sample amounts, technical variation and quantitative lipidomics results for each sample in tabular format. The Extended Report additionally contains a graphical presentation of: (a) averaged lipid class values with replicate standard deviations, (b) averaged (sub)species, double bond, chain length and hydroxylation profiles for each lipid class, (c) principal component analysis for un-averaged replicates. The optional Extended Report can be viewed via internet browser (as an offline local webpage) or from data files that can be browsed manually. Cohorts defined in the sample list associated with analyzed samples are the basis for averaging, visualizing, comparing or any other replicate-dependent statistical proceeding included in the Standard and Extended Report.

Delivery time:
The results will be delivered via email within 4 weeks after receiving the properly prepared samples as well as all information regarding the samples and analysis to be performed. The delivery date of samples to Lipotype will be discussed and agreed between the customer and Lipotype. LIPOTYPE’s obligation to perform under this Quotation shall be suspended as long as and to the extent LIPOTYPE is not able to fully and unrestrainedly use its facilities and personnel due to injunctions, restraints, directions or recommendations (individual or public) by any local, national or international governmental or non-governmental institutions (“Authority Measures”), regardless of whether such Authority Measures affect LIPOTYPE, its directors, employees, agents or subcontractors directly or indirectly.

Quality assurance:
Each analysis includes internal blank and quality control reference samples to assess and improve the technical quality of the analysis. The technical variation of an analytical run assessed from quality control reference samples is provided within the Report. Please note: No further information or data (especially also including individualized data) on blank and quality control reference samples and/or their analysis will be provided to the customer.

Personal support:
Basic consultations and support regarding your analysis and results is included on demand.

Optional features:

• Quantification of free sterols;
• Interactive Data Visualization with LipotypeZoom (available free of charge). LipotypeZoom displays the content of the extended report and is internet-accessible via browser. Lipotype GmbH uploads the results only having the customer’s consent.
• Decreased delivery time for standard and extended report. (2 weeks turbo analysis).